After you add the new Mediation Server to the location profile route, you need to remove the old Mediation Server from the route.
To remove the old Mediation Server from the route
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Open the Office Communications Server 2007 R2 snap-in: Click Start, point to Administrative Tools, and then click Office Communications Server 2007 R2.
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In the console pane, right-click the Forestnode, point to Properties, and then click Voice Properties.
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In Office Communications Server Voice Properties, click the Routetab.
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Select the route you want to modify, and then click Edit.
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In Edit Route, in the Gatewayssection, select the old Mediation Server, and then click Remove.
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In Edit Route, click OK.
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In Office Communications Server Voice Properties, click Apply, and then click OK.